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Part:BBa_K223019:Design

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-07-06)


MCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1659
    Illegal PstI site found at 2224
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1659
    Illegal PstI site found at 2224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1708
    Illegal BglII site found at 2842
    Illegal XhoI site found at 1689
    Illegal XhoI site found at 2745
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1659
    Illegal PstI site found at 2224
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1659
    Illegal PstI site found at 2224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2560


Design Notes

MCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT


Source

MCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT

References